3RA6
Crystal structure of T. celer L30e E62A/K46A variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.587, 53.913, 34.298 |
| Unit cell angles | 90.00, 109.16, 90.00 |
Refinement procedure
| Resolution | 32.398 - 2.000 |
| R-factor | 0.2016 |
| Rwork | 0.196 |
| R-free | 0.25320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h7m |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.008 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.398 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.174 |
| Number of reflections | 5520 | |
| <I/σ(I)> | 15.2 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 5% PEG MME 2000, 0.1M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
