3RA0

Crystal Structure of a StWhy2 K67A-dT32 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.08
Spacegroup name	F 4 3 2
Unit cell lengths	166.465, 166.465, 166.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.427 - 2.451
R-factor	0.2514
Rwork	0.250
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 3N1J
RMSD bond length	0.003
RMSD bond angle	0.705
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.450
Number of reflections	7685
<I/σ(I)>	24.5
Completeness [%]	99.7
Redundancy	17.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	100mM Tris pH8.0, 15% PEG6000, 1200mM LiCl, vapor diffusion, hanging drop, temperature 298K