3R9X

Crystal structure of Era in complex with MgGDPNP, nucleotides 1506-1542 of 16S ribosomal RNA, and KsgA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	195.502, 68.094, 73.587
Unit cell angles	90.00, 100.62, 90.00

Refinement procedure

Resolution	28.686 - 2.800
R-factor	0.1735
Rwork	0.171
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iev 3ftf
RMSD bond length	0.007
RMSD bond angle	1.240
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.097	0.037	0.614
Number of reflections	23217
<I/σ(I)>	9.4	31.89	1.69
Completeness [%]	98.4	99.7	91
Redundancy	4.8	5.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	292	0.1M sodium acetate, 20% (v/v) MPD, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K