3R9R
Structure of a Phosphoribosylaminoimidazole-succinocarboxamide synthase from Mycobacterium abscessus ATCC 19977 / DSM 44196
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.901, 64.786, 48.357 |
| Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
| Resolution | 45.300 - 1.849 |
| R-factor | 0.1773 |
| Rwork | 0.174 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1obg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.391 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.849 | 5.020 | 1.849 |
| Rmerge | 0.082 | 0.032 | 0.369 |
| Number of reflections | 23760 | ||
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 99.9 | 99 | 100 |
| Redundancy | 3.1 | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG3350, Cryo 25% Ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
