3R9J

4.3A resolution structure of a MinD-MinE(I24N) protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-06-22
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 43 21 2
Unit cell lengths	94.393, 94.393, 284.979
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.710 - 4.300
R-factor	0.2947
Rwork	0.294
R-free	0.31060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r9i
RMSD bond length	0.009
RMSD bond angle	1.070
Data scaling software	SCALA (CCP4_3.3.16)
Phasing software	MOLREP
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.530
High resolution limit [Å]	4.300	13.600	4.300
Rmerge	0.108	0.050	0.520
Total number of observations		3960	16272
Number of reflections	9437
<I/σ(I)>	15.3547	39.89	5.49
Completeness [%]	99.9	98.22	100
Redundancy	12.84	10.88	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	293	30% w/v PEG3000, 100 mM CHES, 10 mM EDTA, pH 9.5, VAPOR DIFFUSION, temperature 293K