3R9A
Human alanine-glyoxylate aminotransferase in complex with the TPR domain of human PEX5P
Replaces: 3IMZExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-11-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.367, 99.156, 127.698 |
| Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
| Resolution | 78.090 - 2.350 |
| R-factor | 0.18665 |
| Rwork | 0.184 |
| R-free | 0.23917 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.090 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.115 | |
| Number of reflections | 61118 | |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 292 | 0.1M BIS-TRIS (PH5.3), 0.15M LISO4, 17% PEG 3350, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 292K |
