3R8W

Structure of 3-isopropylmalate dehydrogenase isoform 2 from Arabidopsis thaliana at 2.2 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	76.812, 211.016, 76.903
Unit cell angles	90.00, 90.15, 90.00

Refinement procedure

Resolution	36.998 - 2.250
R-factor	0.177
Rwork	0.175
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.980
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	114991
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.68	298	20% PEG 4000, 20% glycerol, 0.16M ammonium sulfate, 0.08 sodium acetate trihydrate , pH 4.68, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K