3R87

Crystal Structure of Orf6 protein from Photobacterium profundum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2008-06-25
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.0332, 0.9329
Spacegroup name	P 64 2 2
Unit cell lengths	103.370, 103.370, 64.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.440 - 1.050
R-factor	0.14921
Rwork	0.149
R-free	0.16481
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.028
RMSD bond angle	2.162
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.440	1.077
High resolution limit [Å]	1.050	1.050
Number of reflections	88811
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1M MES monohydrate, 1.6M Magnesium sulfate heptahydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K