3R84

Structure of the Mediator head subcomplex Med11/22

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-05-25
Detector	PSI PILATUS 6M
Wavelength(s)	0.9796, 0.9797, 0.9720
Spacegroup name	P 21 21 2
Unit cell lengths	148.490, 173.790, 101.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.040 - 2.050
R-factor	0.1715
Rwork	0.170
R-free	0.20840
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.968
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.050
Rmerge	0.072
Number of reflections	315134
<I/σ(I)>	10.8
Completeness [%]	98.9
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	100 mM MES pH 6, 5.5% PEG 6000 and 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K