3R7W

Crystal Structure of Gtr1p-Gtr2p complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97916
Spacegroup name	P 1 21 1
Unit cell lengths	68.908, 148.439, 98.508
Unit cell angles	90.00, 100.61, 90.00

Refinement procedure

Resolution	44.060 - 2.773
R-factor	0.2382
Rwork	0.236
R-free	0.28310
Structure solution method	SAD
RMSD bond length	0.023
RMSD bond angle	1.521
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.770
Number of reflections	46969
Completeness [%]	95.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1M HEPES, 10% PEG monomethyl ether 5000, 5%(v/v) Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K