3R7F

Crystal Structure of CP-bound Aspartate Transcarbamoylase from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Spacegroup name	C 1 2 1
Unit cell lengths	251.916, 152.960, 51.239
Unit cell angles	90.00, 96.10, 90.00

Refinement procedure

Resolution	34.117 - 2.101
R-factor	0.1709
Rwork	0.171
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.094
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.085	0.047	0.613
Number of reflections	111454
<I/σ(I)>	11.5
Completeness [%]	100.0	99.7	100
Redundancy	7.5	7.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1