3R6B

Crystal Structure of Thrombospondin-1 TSR Domains 2 and 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2011-03-15
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	66.364, 84.817, 60.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.354 - 2.400
R-factor	0.2387
Rwork	0.235
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lsl
RMSD bond length	0.008
RMSD bond angle	1.286
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.354	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.065	0.227
Number of reflections	6564
<I/σ(I)>	16.6	4.7
Completeness [%]	94.0	87.6
Redundancy	5.8	3.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.9M sodium potassium phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K