3R6A
Crystal structure of an uncharacterized protein (hypothetical protein MM_3218) from Methanosarcina mazei.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.433, 62.433, 149.595 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.820 - 1.760 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.880 | 1.810 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.104 | 0.588 |
| Number of reflections | 32561 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 22.5 | 21.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 2.1M ammonium sulfate; 0.1M Na acetate; Trehalose dihydrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
