3R63
Structure of ERK2 (SPE) mutant (S246E)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.770, 69.999, 60.117 |
| Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.19107 |
| Rwork | 0.188 |
| R-free | 0.24473 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.684 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 40979 |
| Completeness [%] | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100mM Bis-Tris buffer, 61mM ammonium Sulfate, 15% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
