3R62
Structure of complement regulator Factor H mutant, T1184R.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.886, 52.840, 142.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.460 - 1.520 |
| R-factor | 0.17922 |
| Rwork | 0.178 |
| R-free | 0.20797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bzm |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.372 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 71.310 | 71.310 | 1.560 |
| High resolution limit [Å] | 1.520 | 6.810 | 1.520 |
| Rmerge | 0.109 | 0.084 | 0.681 |
| Number of reflections | 43524 | ||
| <I/σ(I)> | 11.5 | 22.2 | 2.1 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 6.5 | 5.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 0.1M Tris HCl, pH 8.5 and 12% polyethylene glycol 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 290K |
