3R3T
Crystal Structure of 30S Ribosomal Protein S from Bacillus anthracis
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97942 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 82.680, 82.680, 59.165 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.609 - 2.302 |
R-factor | 0.225 |
Rwork | 0.208 |
R-free | 0.23200 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.384 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: dev_690)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 10636 | |
<I/σ(I)> | 10.3 | 4.65 |
Completeness [%] | 99.8 | 100 |
Redundancy | 12.9 | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 50 mM magnesium chloride, 0.1 M HEPES, 30 % v/v polyethylene glycol monomethyl ether 550, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |