3R2D
Crystal Structure of Antitermination Factors NusB and NusE in complex with dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | MAR 225 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.786, 79.940, 106.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.642 - 2.199 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d3b |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.723 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.1) |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.199 | 4.740 | 2.200 |
| Rmerge | 0.057 | 0.033 | 0.542 |
| Number of reflections | 32257 | ||
| <I/σ(I)> | 14.5 | ||
| Completeness [%] | 94.7 | 87.9 | 94.5 |
| Redundancy | 5.7 | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M diammonium phosphate, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
