3R2C
Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-25 |
Detector | MAR 225 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.530, 113.008, 65.763 |
Unit cell angles | 90.00, 102.87, 90.00 |
Refinement procedure
Resolution | 35.663 - 1.902 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.20180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r2d |
RMSD bond length | 0.007 |
RMSD bond angle | 1.028 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.102 | 0.047 | 0.520 |
Number of reflections | 42602 | ||
<I/σ(I)> | 8.4 | ||
Completeness [%] | 94.2 | 99.5 | 74.5 |
Redundancy | 5 | 5.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 310 | 0.1 M sodium acetate, 25% PEG8000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 310K |