3R29
Crystal structure of RXRalpha ligand-binding domain complexed with corepressor SMRT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2011-01-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.220, 118.220, 84.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.770 - 2.900 |
| R-factor | 0.24425 |
| Rwork | 0.240 |
| R-free | 0.32626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nsp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.814 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.225 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.092 | 0.447 |
| Number of reflections | 13640 | |
| <I/σ(I)> | 49.2 | 7.3 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM sodium cacodylate, 15% PEG4000, 100 mM magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
