3R27
Crystal structure of the first RRM domain of heterogeneous nuclear ribonucleoprotein L (HnRNP L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 |
| Unit cell lengths | 43.170, 43.170, 78.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.250 - 2.040 |
| R-factor | 0.20682 |
| Rwork | 0.204 |
| R-free | 0.25473 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wex |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.112 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.570 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.071 | 0.341 |
| Number of reflections | 10541 | |
| <I/σ(I)> | 14 | 3.7 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 0.1M HEPES, 25%w/v Polyethylene glycol 3,350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
