3R24

Crystal structure of nsp10/nsp16 complex of SARS coronavirus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	67.330, 184.520, 128.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.100 - 2.000
R-factor	0.1945
Rwork	0.193
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.165
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.150
High resolution limit [Å]	2.000	2.000
Rmerge	0.540	0.310
Number of reflections	54053
<I/σ(I)>	12.6	3.1
Completeness [%]	99.2	99
Redundancy	6.1	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	2.0M NaCl, 0.1M NaH2PO4, 0.1M K2HPO4, 0.1M MES, pH5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5	293	2.0M NaCl, 0.1M NaH2PO4, 0.1M K2HPO4, 0.1M MES, pH5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K