3R1O
Odorant Binding Protein 7 from Anopheles gambiae with Four Disulfide Bridges
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.704, 46.469, 57.438 |
| Unit cell angles | 90.00, 103.78, 90.00 |
Refinement procedure
| Resolution | 55.790 - 2.100 |
| R-factor | 0.2735 |
| Rwork | 0.272 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2erb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.800 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.091 | 0.193 |
| Number of reflections | 14055 | |
| <I/σ(I)> | 22.1 | 8.8 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 298 | 33 mM HEPES, pH 7.25-8.25, 0.4-1.0 M KCl, 6.6 mM sodium borate, 0.83 M sodium citrate tribasic, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
