3R0R
The 2.3 A structure of porcine circovirus 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-11 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.88 |
| Spacegroup name | P 1 |
| Unit cell lengths | 193.612, 202.163, 230.975 |
| Unit cell angles | 90.01, 89.33, 90.07 |
Refinement procedure
| Resolution | 39.960 - 2.350 |
| Rwork | 0.225 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 1002451 | |
| Completeness [%] | 68.7 | 44.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 6% PEG 3350, 2.5% isopropanol, 0.3 M monium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
