3R0Q
A Uniquely Open Conformation Revealed in the Crystal Structure of Arabidopsis Thaliana Protein Arginine Methyltransferase 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.552, 86.691, 114.735 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 32.970 - 2.610 |
| R-factor | 0.1846 |
| Rwork | 0.182 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.970 | 2.670 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 46744 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 96.6 | 91 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 295 | 0.1M Tris-HCl, 2.3M Na2HPO4, 0.1M arginine, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
