3R0P
Crystal structure of L-PSP putative endoribonuclease from uncultured organism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97912 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.414, 82.680, 99.311 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.532 - 1.900 |
R-factor | 0.1786 |
Rwork | 0.177 |
R-free | 0.21240 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.056 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.100 | 0.487 |
Number of reflections | 42525 | |
<I/σ(I)> | 29.1 | 2.8 |
Completeness [%] | 99.8 | 98.7 |
Redundancy | 8.8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Hepes, 0.2M Ammonium sulphate, 25% PEG3350, 1/75 subtilisin, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |