3R08

Crystal structure of mouse cd3epsilon in complex with antibody 2C11 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97946
Spacegroup name	I 41 3 2
Unit cell lengths	263.177, 263.177, 263.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.150 - 4.100
R-factor	0.224
Rwork	0.222
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3R06 1sy6
RMSD bond length	0.010
RMSD bond angle	1.440
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.250
High resolution limit [Å]	4.100	4.100
Number of reflections	12153
Completeness [%]	96.9	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.2	298	0.1 M Mes, ammonium sulfate, dioxane, pH 5.2, VAPOR DIFFUSION, temperature 298K