3R06
Crystal structure of anti-mouse CD3epsilon antibody 2C11 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Detector technology | ADSC QUANTUM 315 |
| Collection date | 2006-06-19 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.171, 72.386, 127.487 |
| Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
| Resolution | 36.020 - 2.500 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 62077 | |
| Completeness [%] | 98.6 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | 0.1M HEPES, PEG 4000, pH 7.5, EVAPORATION, temperature 298K |
