3QZ0

Structure of Treponema denticola Factor H Binding protein (FhbB), selenomethionine derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2822
Spacegroup name	P 43 21 2
Unit cell lengths	46.921, 46.921, 168.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.207 - 1.770
R-factor	0.1906
Rwork	0.189
R-free	0.20570
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.038
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE (within PHENIX)
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.770
Number of reflections	19167
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	1.39 M sodium citrate, 0.1 M HEPES, 0.2 M sodium thiocyanate, 10 mM dithiothreitol, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K