3QYN

Structure of p63 DNA Binding Domain in Complex with a 22 Base Pair A/T Rich Response Element Containing 2 Base Pair Spacer Between Half Sites

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-03
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 63
Unit cell lengths	140.984, 140.984, 119.346
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.048 - 2.500
R-factor	0.2121
Rwork	0.210
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qym
RMSD bond length	0.011
RMSD bond angle	1.635
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.346	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	46625
<I/σ(I)>	20.1	3.6
Completeness [%]	99.9	100
Redundancy	12.8	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	20% PEG3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K