3QXW
Free structure of an anti-methotrexate CDR1-4 Graft VHH Antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-12 |
| Detector | MAR 225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.043, 59.167, 131.425 |
| Unit cell angles | 90.00, 92.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.19163 |
| Rwork | 0.189 |
| R-free | 0.23828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.540 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.089 |
| Number of reflections | 50632 |
| Completeness [%] | 99.5 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2M Lithium Sulfate Monohydrate, 0.1M BIS-TRIS, 25% PEG 3000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
