3QWR
Crystal structure of IL-23 in complex with an adnectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-31 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 77.700, 91.700, 225.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.480 - 3.250 |
| R-factor | 0.235 |
| Rwork | 0.234 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IL-23 FROM UNPUBLISHED STRUCTURE AND ADNECTIN FROM PDB ENTRY 1FNF RESIDUES 1421-1437 1444-1466 1470-1491 1502-1509 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.13.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.480 | 3.370 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.096 | 0.320 |
| Number of reflections | 12835 | |
| <I/σ(I)> | 7.6 | 3.2 |
| Completeness [%] | 98.0 | 99.5 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 0.1 M Tris-HCl, 1 M tri-sodium citrate, 0.2 M NaCl, pH 7.0, vapor diffusion, hanging drop, temperature 293K |
