3QWP
Crystal structure of SET and MYND domain containing 3; Zinc finger MYND domain-containing protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.303, 66.206, 107.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.390 - 1.530 |
| R-factor | 0.17655 |
| Rwork | 0.174 |
| R-free | 0.21665 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mek |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.507 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.074 | 0.564 |
| Number of reflections | 66393 | |
| <I/σ(I)> | 30.3 | 1.7 |
| Completeness [%] | 99.3 | 92.3 |
| Redundancy | 8.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 20% PEG 3350, 0.2M CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
