3QWG
Crystal structure of EspRdelta10, C-terminal 10 amino acids deletion mutant of EspR transcription factor from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0097 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.460, 55.370, 76.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.860 - 1.992 |
R-factor | 0.1852 |
Rwork | 0.183 |
R-free | 0.23560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qf3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.945 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CCP4 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.860 | 2.110 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.104 | 0.387 |
Number of reflections | 24707 | |
<I/σ(I)> | 9.83 | 3.11 |
Completeness [%] | 82.6 | |
Redundancy | 3.4 | 3.02 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 20% PEG 4000, 100mM Tris-HCl, 200 mM LiSO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |