3QVR

Crystal structure of glucose oxidase for space group P3121 at 1.3 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	P 31 2 1
Unit cell lengths	65.952, 65.952, 214.252
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.060 - 1.300
R-factor	0.18
Rwork	0.180
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cf3
RMSD bond length	0.014
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (from ccp4)
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	1.460	1.320
High resolution limit [Å]	1.300	1.430	1.300
Rmerge	0.056	0.233	0.321
Number of reflections	132764
<I/σ(I)>	29.9	6.1	3.6
Completeness [%]	99.2	99.3	98.7
Redundancy	8	6.6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	295	200 mM CaCl2 and 20% PEG 3350, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K