3QVE
Crystal structure of human HMG box-containing protein 1, HBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-30 |
| Detector | ADSC Q315 3x3 CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 118.840, 118.840, 93.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.900 - 2.040 |
| R-factor | 0.1637 |
| Rwork | 0.162 |
| R-free | 0.19175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v06 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.557 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.420 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.097 | 0.757 |
| Number of reflections | 48353 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 5.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 25% PEG 3350, 0.3M Ammonium sulfate, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
