3QUS
Crystal Structure of N10-Formyltetrahydrofolate Synthetase with ATPgS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 160.840, 160.840, 256.479 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 122.400 - 2.840 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.28860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3PZX |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.657 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 122.405 | 2.890 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.148 | 0.490 |
| Number of reflections | 30394 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 97.0 | 99.8 |
| Redundancy | 11.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 50-75 mM Potassium Maleate Buffer pH 7.0-8.0, 1 mM dithiothreitol, 38-46% Ammonium Sulfate, 1-3.5% PEG 1000 or PEG 1450, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
