3QUK
Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with LCMV-derived gp33 altered peptide ligand (Y4A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.007 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.510, 126.210, 96.470 |
| Unit cell angles | 90.00, 124.54, 90.00 |
Refinement procedure
| Resolution | 33.900 - 2.410 |
| R-factor | 0.2439 |
| Rwork | 0.242 |
| R-free | 0.28490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7u |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.192 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.052 | 0.213 |
| Number of reflections | 44615 | |
| <I/σ(I)> | 20 | 4.7 |
| Completeness [%] | 98.9 | 81 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.8 M ammonium sulfate, 0.1 M Tris HCl pH 9.0., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
