3QU3
Crystal structure of IRF-7 DBD apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2008-08-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 31 |
| Unit cell lengths | 68.588, 68.588, 68.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.700 - 1.300 |
| R-factor | 0.169 |
| Rwork | 0.161 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o61 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.077 | 0.398 |
| Number of reflections | 85736 | |
| <I/σ(I)> | 21.3 | 3.4 |
| Completeness [%] | 99.8 | 94.2 |
| Redundancy | 6.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 19% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
