3QTK
The crystal structure of chemically synthesized VEGF-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 230.922, 44.008, 73.111 |
| Unit cell angles | 90.00, 99.93, 90.00 |
Refinement procedure
| Resolution | 32.509 - 1.849 |
| R-factor | 0.1825 |
| Rwork | 0.180 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vpf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_624) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.849 | 3.990 | 1.850 |
| Rmerge | 0.074 | 0.040 | 0.574 |
| Number of reflections | 61902 | ||
| <I/σ(I)> | 17.98 | 1.85 | |
| Completeness [%] | 99.2 | 96.7 | 99.8 |
| Redundancy | 3.1 | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 45% v/v MPD, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
