3QTA

Crystal structure of a CheC-like protein (rrnAC0528) from Haloarcula marismortui ATCC 43049 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-27
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97958,0.97885
Spacegroup name	P 41 21 2
Unit cell lengths	89.928, 89.928, 148.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.976 - 2.000
R-factor	0.1762
Rwork	0.175
R-free	0.20230
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.488
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.976	29.976	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge		0.011	0.011
Total number of observations		3346	22338
Number of reflections	41896
<I/σ(I)>	8.9	25.8	2
Completeness [%]	100.0	97.5	100
Redundancy	7.3	6	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.16M Ca(OAc)2, 20.0% Glycerol, 14.4% PEG-8000, 0.1M Cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K