3QSY
Recognition of the methionylated initiator tRNA by the translation initiation factor 2 in Archaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 198.3 |
| Detector technology | CCD |
| Collection date | 2008-10-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 93.680, 93.680, 220.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.950 - 3.200 |
| R-factor | 0.2786 |
| Rwork | 0.278 |
| R-free | 0.30250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aho |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.307 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.400 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.300 | 3.000 |
| Number of reflections | 23068 | ||
| Completeness [%] | 99.6 | 100 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 45% ammonium sulphate, 30mM Tris-HCl, 5mM MES, 7mM MgCl2, 0.5mM DTT, 0.2mM GDPNP, 300mM NDSB 195, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K |
