3QSV
Structural basis for DNA recognition by constitutive Smad4 MH1 dimers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Detector technology | CCD |
| Collection date | 2010-09-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.357, 35.354, 139.544 |
| Unit cell angles | 90.00, 93.83, 90.00 |
Refinement procedure
| Resolution | 24.890 - 2.708 |
| R-factor | 0.2153 |
| Rwork | 0.212 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.926 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 24301 |
| Completeness [%] | 97.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 291.15 | 200mM MgCl2, 100mM Tris, 30% PEG 4000, 10mM spermine, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
