3QS7
Crystal structure of a human Flt3 ligand-receptor ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.890, 146.260, 105.950 |
| Unit cell angles | 90.00, 109.66, 90.00 |
Refinement procedure
| Resolution | 39.040 - 4.300 |
| R-factor | 0.2614 |
| Rwork | 0.260 |
| R-free | 0.28120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1ETE AND 2E9W |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.450 |
| High resolution limit [Å] | 4.300 | 4.300 |
| Rmerge | 0.108 | 0.759 |
| Number of reflections | 20184 | |
| <I/σ(I)> | 12.04 | 2.08 |
| Completeness [%] | 98.8 | 98.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 11-13% PEG4000, 0.1 M magnesium chloride, 50 mM MES, 5 mg/mL Flt3 ligand-receptor complex, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
