3QRL
Crystal Structure of the Taf14 YEATS domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.019, 113.019, 26.329 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.626 - 1.700 |
| R-factor | 0.18988 |
| Rwork | 0.188 |
| R-free | 0.23508 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.516 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.626 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.032 | 0.408 |
| Number of reflections | 21602 | |
| <I/σ(I)> | 24.3 | 3.4 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 42.5% (v/v) PEG-600, 100 mM Sodium Citrate pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
