3QR7

Crystal structure of the C-terminal fragment of the bacteriophage P2 membrane-piercing protein gpV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-12-16
Detector	PSI PILATUS 6M
Spacegroup name	H 3 2
Unit cell lengths	49.069, 49.069, 463.896
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.327 - 0.940
R-factor	0.1025
Rwork	0.102
R-free	0.11200
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.991
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX (C/D/E)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.330	0.990
High resolution limit [Å]	0.940	0.940
Rmerge	0.063	0.282
Number of reflections	141144
<I/σ(I)>	16.6	5.8
Completeness [%]	99.9	99.9
Redundancy	6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	50 mg/mL protein, 30% PEE 797, 0.3 M ammonium sulfate, 0.1 M MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K