3QR5
Structure of the first domain of a cardiac Ryanodine Receptor mutant with exon 3 deleted
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9729 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.370, 44.204, 76.606 |
| Unit cell angles | 90.00, 90.98, 90.00 |
Refinement procedure
| Resolution | 38.290 - 2.300 |
| R-factor | 0.2109 |
| Rwork | 0.209 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.435 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.065 | 0.048 | 0.200 |
| Number of reflections | 12917 | ||
| <I/σ(I)> | 14 | ||
| Completeness [%] | 84.5 | 98.8 | 32.9 |
| Redundancy | 1.8 | 2 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10-15% PEG 1000. Crystals frozen in 25% 2-methane-2,4-pentanediol cryoprotectant, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
