3QR0
Crystal Structure of S. officinalis PLC21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-11-02 |
| Detector | Rayonix MarMosiac 225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.815, 89.396, 163.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.360 - 2.000 |
| R-factor | 0.17754 |
| Rwork | 0.176 |
| R-free | 0.20819 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 2ZKM |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.946 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.890 | 5.130 | 1.890 |
| Rmerge | 0.069 | 0.032 | |
| Number of reflections | 70063 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 97.7 | 98.6 | 97.5 |
| Redundancy | 3.7 | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | 100 mM Bis-Tris, 100 mM NaCl, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
