3QQN

The retinal specific CD147 Ig0 domain: from molecular structure to biological activity

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-28
Detector	NOIR-1
Wavelength(s)	1.02159,1.02195,1.03726
Spacegroup name	P 65 2 2
Unit cell lengths	80.240, 80.240, 160.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.420 - 2.309
R-factor	0.238
Rwork	0.234
R-free	0.26860
RMSD bond length	0.009
RMSD bond angle	1.353
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.420
High resolution limit [Å]	2.309
Number of reflections	14020
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.2M Ammonium Sulfate, 20% PEG 3350, 0.1M spermidine tetrachloride, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K