3QQM

Crystal structure of a Putative amino-acid aminotransferase (NP_104211.1) from Mesorhizobium loti at 2.30 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-20
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	116.178, 215.574, 81.875
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.683 - 2.300
R-factor	0.1956
Rwork	0.193
R-free	0.24070
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.461
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.683	29.683	2.380
High resolution limit [Å]	2.300	4.930	2.300
Rmerge	0.084	0.031	0.428
Number of reflections	91682	17591	16526
<I/σ(I)>	8.97	22	1.8
Completeness [%]	98.6	97.9	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	277	0.200M NH4I, 20.00% PEG-3350, No Buffer pH 6.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K