3QQ7

Crystal Structure of the p97 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 31
Unit cell lengths	60.560, 60.560, 63.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.280 - 2.650
R-factor	0.1967
Rwork	0.195
R-free	0.23550
RMSD bond length	0.012
RMSD bond angle	1.351
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	PHASER
Refinement software	PHENIX (1.6.2_432)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.446	31.555	2.790
High resolution limit [Å]	2.650	8.380	2.650
Rmerge	0.050	0.021	0.573
Number of reflections	7515
<I/σ(I)>	16.4	23.3	1.3
Completeness [%]	100.0	98.6	100
Redundancy	4.5	4.4	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	1 M Hexanediol, 0.1 M Na-acetate, 0.01 M CoCl2, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K